BSL2.3 Laboratory

The BSL2.3 laboratory  is designed for the generation of plasmid vectors for ectopic expression of genes of interest: PCR amplification of the genes, enzymatic digestion of DNA using specific restriction enzymes, agarose gel electrophoresis of nucleic acids, cloning of nucleic acid sequences with the generation of specific plasmids and transformation of competent bacteria for plasmid production, bacterial cultures in liquid and solid medium, using both static incubator and shaker and agitator, isolation of plasmids from bacterial cultures.